ʻO nā mea hoʻohālikelike kumu ulu i loaʻa i ka platelet SEB-C100
Hoʻolauna huahana
ʻO ka Platelet Derived Growth Factor Analyzer kahi mea hoʻāʻo a hoʻopaʻa ʻia e pili ana i kahi ʻano hoʻokolohua kūʻokoʻa i paionia e kā mākou hui.ʻIke ʻia ka mea anamanaʻo i ka mea ulu i loaʻa i ka platelet, kahi hōʻailona protein kikoʻī i loko o ka mimi kanaka i hana ʻia i ka wā e kū mai ai ka stenosis coronary artery.Hiki ke hoʻopau ʻia ka loiloi i loko o kekahi mau minuke me ka hoʻohana ʻana i 1ml o ka mimi.Hiki i ka mea loiloi ke hoʻoholo inā loaʻa ka stenosis a me ke ʻano o ka stenosis i mea e hāʻawi ai i kahi kuhikuhi no ka nānā hou ʻana.ʻO ke ala ʻike a me ka nānā ʻana o ka platelet-derived growth factor analyzer he kumu ʻike ʻole invasive ʻole, ʻaʻole ia e koi i nā injections a me nā lāʻau kōkua, e hoʻopau i ka pilikia i hiki ʻole i ka poʻe maʻi i ka iodine-containing contrast agents ke hana i ka CT a me nā coronary ʻē aʻe. angiography aʻaʻa.Loaʻa i ka mea anamanaʻo nā mea maikaʻi o ke kumukūʻai hoʻāʻo haʻahaʻa, ākea o ka noi, noi maʻalahi, wikiwiki hoʻāʻo wikiwiki, a me nā mea ʻē aʻe, a he ʻano hou o ka coronary artery stenosis ka ʻike mua ʻana a me ka mea nānā.
Loaʻa i ka analyzer nā pono penei:
1. Ka wikiwiki: E hoʻokomo i ka mimi i loko o ka mea ʻike a kali wale i kekahi mau minuke
2. ʻoluʻolu: ʻAʻole loaʻa ka hoʻāʻo ʻana i nā halemai.Hiki ke hana ʻia ma nā keʻena hoʻopaʻa olakino, nā hale hānai a i ʻole nā home kōkua kaiāulu
3. Hōʻoluʻolu: ʻO 1ml wale nō o ka mimi e pono ai ma ke ʻano he laʻana, ʻaʻohe huki koko, ʻaʻohe lāʻau lapaʻau, ʻaʻohe ʻokoʻa ʻokoʻa, ʻaʻohe hopohopo e pili ana i nā hopena maʻi.
4. Naʻauao: Fully automated inspection, working on unttended
5. Hoʻopili maʻalahi: ʻO ka liʻiliʻi liʻiliʻi, hiki ke hoʻokomo a hoʻohana ʻia me ka hapalua papaʻaina
6. Mālama maʻalahi: E nānā pono a hōʻike i ke kūlana consumable no ka hoʻololi maʻalahi
Ke kumu o ka huahana
Hoʻohana ʻo Raman spectroscopy i ka hoʻopuehu māmā e hoʻopaʻa wikiwiki i ke ʻano molekala.Hoʻokumu ʻia kēia ʻenehana i ka manaʻo i ka wā e hoʻomālamalama ai ka mālamalama i kahi mole, e kū mai nā huila elastic a hoʻopuehu kekahi hapa o ka māmā.ʻOkoʻa ka alapine o ka mālamalama i hoʻopuehu ʻia mai ke alapine o ka mālamalama hanana, i kapa ʻia ʻo Raman scattering.Hoʻopili ka ikaika o ka hoʻopuehu ʻana o Raman me ke ʻano o ka mole, e ʻae ana i ka nānā ʻana i kona ikaika a me ka alapine e hoʻoholo pololei i ke ʻano a me ke ʻano o ka mole.
Ma muli o ka hōʻailona Raman nāwaliwali a me ka hoʻopili pinepine ʻana i ka fluorescence, hiki ke paʻakikī ka loaʻa ʻana o Raman spectra i ka wā ʻike maoli.Paʻakikī maoli ka ʻike ʻana i ka hōʻailona Raman.No laila, hiki ke hoʻonui nui i ka spectroscopy Raman i hoʻonui ʻia i ka ikaika o ka mālamalama hoʻopuehu Raman, e hoʻoponopono i kēia mau pilikia.ʻO ke kumu kumu o ka ʻenehana ke kau ʻana i ka mea e ʻike ʻia ma luna o kahi ʻili metala kūikawā, e like me ke kālā a i ʻole ke gula.i mea e hana ai i ka ili okea, nanometer-level, ka hopena o ka hoonui i ka ili.
Ua hōʻike ʻia ʻo ka Raman spectrum o ka marker platelet-derived growth factor (PDGF-BB) i hōʻike ʻia i kahi piko ʻokoʻa ma 1509 cm-1.Eia hou, ua hoʻopaʻa ʻia ʻo ka hele ʻana o ka marker platelet-derived growth factor (PDGF-BB) i loko o ka mimi i hoʻopili ʻia me ka stenosis coronary artery.
Ma ka hoʻohana ʻana i ka ʻenehana Raman spectroscopy a me ka ʻenehana hoʻonui ʻana i ka ʻili, hiki i ka mea hoʻoponopono PDGF ke ana i ka hele ʻana o PDGF-BB a me kona ʻano kiʻekiʻe kiʻekiʻe i ka mimi.ʻO kēia ka mea e hiki ai ke hoʻoholo inā he stenotic nā aʻa coronary a me ke kiʻekiʻe o ka stenosis, no laila ke hāʻawi nei i kumu no ka maʻi maʻi.
Ka hope o ka huahana
I nā makahiki i hala iho nei, ua piʻi mālie ka nui o ka maʻi naʻau coronary ma muli o ka hoʻololi ʻana i ka meaʻai a me nā ʻano nohona, a me ka heluna kanaka ʻelemakule.ʻO ka nui o ka make e pili ana i ka maʻi naʻau coronary ke mau nei ka weliweli.Wahi a ka China Cardiovascular Health and Disease Report 2022, ʻo ka nui o ka make o ka maʻi naʻau coronary ma waena o nā kamaʻāina Kina he 126.91/100,000 a me 135.88/100,000 ma waena o nā kamaʻāina kuaʻāina i 2020. Ua piʻi ka helu mai ka makahiki 2012 me ka piʻi nui ma kuaaina.Ma 2016, ua ʻoi aku ia ma mua o ka pae o ke kūlanakauhale a hoʻomau i ka piʻi ʻana ma 2020. I kēia manawa, ʻo ka coronary arteriography ke ʻano diagnostic mua i hoʻohana ʻia i nā hoʻonohonoho maʻi e ʻike ai i ka maʻi naʻau coronary.ʻOiai i kapa ʻia ʻo "gold standard" no ka maʻi maʻi naʻau coronary, ʻo kona invasiveness a me ke kumukūʻai kiʻekiʻe ua alakaʻi i ka hoʻomohala ʻana o ka electrocardiography ma ke ʻano he ʻano hana diagnostic ʻē aʻe.ʻOiai he maʻalahi ka maʻi electrocardiogram (ECG), maʻalahi, a maʻalahi hoʻi, hiki ke hana ʻia nā kuhi hewa a me nā haʻalele ʻana o nā maʻi, ʻaʻole hiki ke hilinaʻi ʻia no ka maʻi maʻi o ka maʻi naʻau coronary.No laila, ʻo ka hoʻomohala ʻana i kahi ala ʻaʻole invasive, koʻikoʻi, a hilinaʻi hoʻi no ka ʻike mua a me ka wikiwiki o ka maʻi naʻau coronary he mea nui.
Ua loaʻa i ka Surface-enhanced Raman spectroscopy (SERS) ka hoʻohana ākea i ka ʻepekema ola no ka ʻike ʻana i nā biomolecules ma nā haʻahaʻa haʻahaʻa loa.No ka laʻana, ʻo Alula et al.Ua hiki iā lākou ke ʻike i ke kiʻekiʻe o ka creatinine i loko o ka mimi me ka hoʻohana ʻana i ka spectroscopy SERS me ke kiʻi catalytically modified nanoparticles kala i loaʻa nā mea magnetic.
Pēlā nō, ʻo Ma et al.hoʻohana i ka hoʻohui magnetically induced o nā nanoparticles ma SERS spectroscopy e hōʻike i ka haʻahaʻa haʻahaʻa loa o ka deoxyribonucleic acid (DNA) i loko o ka bacteria.
He kuleana koʻikoʻi ka platelet-derived growth factor-BB (PDGF-BB) i ka hoʻomohala ʻana i ka atherosclerosis ma o nā ʻano hana he nui a pili loa i ka maʻi naʻau coronary.ʻO ka Enzyme-linked immunosorbent assay (ELISA) ka hana nui i hoʻohana ʻia i ka noiʻi PDGF-BB o kēia manawa no ka ʻike ʻana i kēia protein i loko o ke kahe koko.No ka laʻana, ua hoʻoholo ʻo Yuran Zeng a me nā hoa hana i ka neʻe ʻana o ka plasma o PDGF-BB ma o ka hoʻohana ʻana i ka enzyme-linked immunosorbent assay a ʻike i ka PDGF-BB i hāʻawi nui i ka pathogenesis o carotid atherosclerosis.Ma kā mākou noiʻi ʻana, ua ʻimi mua mākou i ka SERS spectra o nā ʻano wai wai PDGF-BB me nā haʻahaʻa haʻahaʻa loa, me ka hoʻohana ʻana i kā mākou 785 nm Raman spectroscopy platform.Ua ʻike mākou ua hāʻawi ʻia nā kiʻekiʻe hiʻohiʻona me ka hoʻololi ʻana o Raman o 1509 cm-1 i ka hoʻonā wai o PDGF-BB.Hoʻohui ʻia, ua ʻike mākou ua pili pū ʻia kēia mau hiʻohiʻona ʻano me ka hoʻonā wai o PDGF-BB.
Ua hui pū kā mākou hui me nā hui noiʻi o ke kulanui e hana i ka nānā ʻana o SERS spectroscopy ma ka huina o 78 mau laʻana mimi.ʻO kēia mau mea he 20 mau laʻana mai nā poʻe maʻi i hana i ka ʻoki ʻana i ka PCI, 40 mau laʻana mai nā maʻi i hana ʻole i ka ʻoki ʻana i ka PCI, a me 18 mau laʻana mai nā kānaka olakino.Ua nānā pono mākou i ka mimi SERS spectra ma ka hoʻohui ʻana i nā kiʻekiʻe Raman me ka hoʻololi pinepine ʻana o Raman o 1509cm-1, i pili pono iā PDGF-BB.Ua hōʻike ʻia ka noiʻi ʻana i nā ʻano mimi o nā mea maʻi i hana i ka ʻoki ʻana i ka PCI he 1509cm-1 kahi kiʻekiʻe i ʻike ʻia, ʻoiai ʻaʻole i loaʻa kēia kiʻekiʻe i nā hōʻailona mimi o nā kānaka olakino a me ka hapa nui o nā mea maʻi ʻole PCI.I ka manawa like, i ka wā i hui pū ʻia ai ka ʻikepili o ka haukapila o ka coronary angiography, ua hoʻoholo ʻia e pili pono kēia ʻano ʻike me ka hoʻoholo inā loaʻa kahi blockage cardiovascular ma mua o 70%.Eia kekahi, hiki i kēia ʻano ke ʻike me ka sensitivity a me ka kikoʻī o 85% a me 87% i kēlā me kēia, ke ʻano o ka blockage i ʻoi aku ma mua o 70% i nā hihia o ka maʻi coronary artery ma ka ʻike ʻana i nā hiʻohiʻona o Raman o 1509 cm-1.5%, no laila, ua manaʻo ʻia e lilo kēia ala i kumu koʻikoʻi no ka hoʻoholo ʻana inā pono nā poʻe maʻi me ka maʻi coronary artery i ka PCI, e hāʻawi ana i nā ʻike maikaʻi loa no ka ʻike mua ʻana i nā hihia i manaʻo ʻia o ka maʻi ʻaʻaʻa coronary.
Ma muli o kēia ʻano, ua hoʻokō kā mākou hui i nā hopena o kā mākou noiʻi mua ma o ka hoʻolaha ʻana i ka Platelet Derived Growth Factor Analyzer.E hoʻololi nui kēia mea hana i ka hoʻolaha ʻana a me ka hoʻohana nui ʻana o ka ʻike mua ʻana i ka maʻi naʻau coronary.E kōkua nui ia i ka hoʻomaikaʻi ʻana i ke olakino o ka naʻau coronary ma Kina a me ka honua.
Palapala puke
[1] Huinan Yang, Chengxing Shen, Xiaoshu Cai et al.Noinvasive a me ka prospective diagnostics o ka maʻi coronary puʻuwai me ka mimi e hoʻohana ana i ka surface-enhanced Raman spectroscopy [J].Heluhelu, 2018, 143, 2235–2242.
Pepa palena
| helu kumu hoʻohālike | SEB-C100 |
| mea hoao | ʻO ka ikaika o ka platelet i loaʻa i ka ulu ʻana i nā hiʻohiʻona kiʻekiʻe i ka mimi |
| Nā ʻano hoʻāʻo | mīkini hana |
| ʻŌlelo | Pākē |
| Kumu ʻike | Raman spectroscopy |
| pāʻani kamaʻilio | Puka USB Micro, Puka Pūnaewele, WiFi |
| hiki ke hana hou | Coefficient o ka hoʻololi ʻana o nā hopena hoʻāʻo ≤ 1.0% |
| degere o ka pololei | Kūlike nā hualoaʻa me nā kumu hoʻohālike o nā kūlana kūpono. |
| kūpaʻa | Coefficient o ka hoʻololi ≤1.0% no ka laʻana like i loko o 8 mau hola o ka mana |
| ʻAno hoʻopaʻa | Hōʻike LCD, mālama ʻikepili FlashROM |
| manawa ʻike | ʻO ka manawa ʻike no ka laʻana hoʻokahi ma lalo o 120 kekona |
| Mana Hana | mea hoʻopili mana: AC 100V~240V， 50/60Hz |
| nā ana waho | 700mm（L)*560mm(W)*400mm(H) |
| kaumaha | Ma kahi o 75 kg |
| kaiapuni hana | ka mahana hana: 10 ℃ ~ 30 ℃;haʻahaʻa pili: ≤90%;kaomi ea: 86kPa～106kPa |
| ʻO ka halihali a me ke kaiapuni mālama | mahana hana: -40 ℃ ~ 55 ℃;haʻahaʻa pili: ≤95%;kaomi ea: 86kPa～106kPa |
